Hairpin ribozyme cleavage catalyzed by aminoglycoside antibiotics urophiliacs the polyamine spermine in the absence of metal ions.
The bolded and underlined Gs were mutated to Cs for the inactive mutant.
Shown as best fits to Eq.
Fraction of substrate bound at indicated enzyme concentration was determined by quantification of the bands ImageJ.
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Error bars represent S.
We first sought to understand how the chemical identity of polyamines that form complex coacervates affects template-directed polymerization of RNA with activated nucleotides Fig.